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| Registros recuperados: 16 | |
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| Riaz,Saba; Bashir,Muhammad Faisal; Haider,Saleem; Rahid,Naeem. |
| Abstract The presented study had two objectives. The first was to examine distributions of Hepatitis C Virus (HCV) genotypes in Sindh, Pakistan, where HCV is prevalent. The other was to explore clinically relevant relationships between the genotypes, viral load (measured by real-time polymerase chain reaction assays) and biochemical markers. For this, 1471 HCV-infected patients in six cities in Sindh were recruited and sampled. HCV genotype distributions varied among the cities, but genotype 3a was most prevalent, followed by 3b, 1a and 1b (detected in 51.5, 22.7. 9.25 and 3.2% of the cases, respectively). No type-specific sequences were detected in serum samples from 189 (12.8%) of the 1471 patients. Frequencies of low (<200,000 IU/mL serum),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sindh; HCV; Pakistan; Genotype; Biomarkers; Viral load. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400980 |
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| Fellner,María Dolores; Durand,Karina; Rodriguez,Marcelo; Irazu,Lucía; Alonio,Virginia; Picconi,María Alejandra. |
| INTRODUCTION: The quantification of circulating Epstein-Barr virus (EBV) DNA is used to monitor transplant patients as an early marker of Post-Transplant Lymphoproliferative Disorders (PTLD). So far no standardized methodology exists for such determination.OBJECTIVE: Our purpose was to develop and validate a real-time PCR assay to quantify EBV DNA in clinical samples from transplant recipients.METHODS: A duplex real-time PCR method was developed to amplify DNA from EBV and from a human gene. The EBV load was determined in peripheral blood mononuclear cells (PBMC), plasma and oropharyngeal tissue from 64 non-transplanted patients with lymphoid-hypertrophy (Non-Tx), 47 transplant recipients without PTLD (Tx), 54 recipients with PTLD (Tx-PTLD), and 66 blood... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EBV; Real-time PCR; Viral load; PTLD. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300271 |
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| Alves,Fabiana Aparecida; Campos,Karoline Rodrigues; Lemos,Marcílio Figueiredo; Moreira,Regina Célia; Caterino-de-Araujo,Adele. |
| ABSTRACT Co-infections of hepatitis C virus (HCV) and either human immunodeficiency virus type 1 (HIV-1), human T-cell lymphotropic virus type 1 (HTLV-1) or type 2 (HTLV-2) have been described as having an impact on HCV viremia and subsequent disease progression. HCV load in serum samples from 622 patients (343 males, 279 females; median age 50.8 years) from São Paulo/southeast Brazil was analyzed using the Abbott Real Time HCV assay (Abbott Molecular Inc., IL, USA). Samples were obtained from HCV-monoinfected (n = 548), HCV/HIV-1- (n = 41), HCV/HTLV-1- (n = 16), HCV/HTLV-2- (n = 8), HCV/HIV/HTLV-1- (n = 4), and HCV/HIV/HTLV-2-co-infected (n = 5) patients, and results were compared among the groups and according to sex. The median HCV load in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HCV; HIV-1; HTLV-1: HTLV-2; Co-infection; Viral load. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200123 |
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| Senise,J.F.; Palacios,R.; Tanno,Z.N.; Lunardi,L.; Waghabi,G.R.; Vaz,M.J.R.; Diaz,RS.; Castelo,A.. |
| It is currently recommended that antiretroviral prophylaxis to prevent mother-to-child transmission (MTCT) of HIV be initiated at 14 weeks of gestation. However, the relevance of early-gestation HIV viral load level for intrauterine MTCT is unknown. The objective of this study was to determine the relationship between prenatal maternal viral load and intrauterine MTCT. Records of HIV-infected pregnant women in two centers in Brazil, from 1999 to 2004 were analyzed. Three pregnancy periods were considered: earlier than 14 weeks, 14 to 27 6/7 weeks, and 28 weeks of gestation or more. Peripartum HIV exposure was also computed. Maximum viral load in each period was the measure of HIV exposure. Four hundred fifty-seven HIV-infected pregnant women were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1; Disease transmission; Vertical transmission; Antiretroviral therapy; Highly active; Brazil; Viral load. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000400009 |
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| Vera-Uehara,Carmina; Sánchez-Alemán,Miguel Angel; Uribe-Salas,Felipe Javier; Ramos-Castañeda,José; Olamendi-Portugal,Ma. Leónidez; Conde-Glez,Carlos Jesús. |
| Objectives:To determine the prevalence of HPV and the risky sexual behaviors associated to it in a sample of male college students, taking into account genotype and viral load.Methods:From 2002 to 2003, male students from the Autonomous University of Morelos State completed a questionnaire and provided self-collected genital samples to detect and quantify HPV. We performed a bivariate and a multivariate logistic regression analysis to identify correlates associated with the infection and to assess the viral load as a function of the viral infecting type. The fragments of β-globin gene and L1 of HPV, were amplified, purified and cloned, to evaluate viral load.Results:Among 253 subjects, HPV prevalence was 19.4%, and HPV16 was the most common subtype.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HPV; Viral load; Contraceptive pills; Risky sexual behaviors; Male; Students. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000100071 |
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| Almeida,Sergio Monteiro de; Letendre,Scott; Ellis,Ron. |
| The pandemic of HIV/AIDS continues to grow daily. Incident cases among women, intravenous drug users and ethnic minorities comprise the fastest growing segment of the HIV-infected population, and the number of HIV-infected individuals over the age of 50 is growing rapidly. Today, the central nervous system and the immune system are seen as main targets of HIV infection. Significant progress in the knowledge and treatment of AIDS has been obtained in recent years. The neurological manifestations directly related to HIV are acute viral meningitis, chronic meningitis, HIV-associated dementia (HAD), vacuolar myelopathy, and involvement of the peripheral nervous system. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV; AIDS; HIV dementia; Central nervous system; Cerebrospinal fluid; Viral load; Chemokines. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000100009 |
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| Levi,José E.; Fink,Maria C.S.; Canto,Cynthia L.M.; Carretiero,Nadily; Matsubara,Regina; Linhares,Iara; Dores,Gérson B. das; Castelo,Adauto; Segurado,Aluísio; Uip,David E.; Eluf Neto,José. |
| HIV-infected women from São Paulo city were enrolled in a cross-sectional study on Human Papillomavirus (HPV) and cervical intraepithelial neoplasia (CIN) prevalence and their association with laboratory markers of AIDS, namely HIV viral load and CD4+ cell counts. A cervical specimen was collected and submitted to Hybrid Capture, a test for HPV viral load determination. HPV-DNA was detected in 173 of 265 women (64.5%). Twenty (7.5%) women were infected by one or more low-risk viruses, 89 (33%) by one or more high-risk viruses, and 64 (24%) harbored at least one HPV type from each risk group. Abnormal smears were observed in 19% of the patients, though there were no invasive carcinomas. Severely immunosuppressed patients (CD4/µL <100) were at the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human Papillomavirus; Cervical intraepithelial neoplasia; HIV-infected women; Viral load; AIDS. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000300005 |
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| Slavov,Svetoslav Nanev; Kashima,Simone; Silva-Pinto,Ana Cristina; Amarilla,Alberto Anastacio; Aquino,Victor Hugo; Covas,Dimas Tadeu. |
| Human parvovirus B19 is a well-known cause of severe conditions in patients with sickle cell disease, but the molecular mechanisms of the infection are insufficiently understood. The different clinical outcome of the acute parvovirus B19 infection in two pediatric patients with sickle cell disease has been examined. One of them developed life-threatening condition requiring emergency transfusions, while the other had asymptomatic infection, diagnosed occasionally. Both cases had high viral load and identical subgenotype, indicating that the viral molecular characteristics play a minimal role in the infection outcome. |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Human parvovirus B19; Viral load; Aplastic crisis; Subgenotypes. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000100017 |
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| Caballero,O.L.; Costa,M.C.S.L.; Trevisan,A.; Oliveira,R.M.; Viotti,E.A.; Távora,E.R.F.; Vilaça,S.S.; Sabagga,E.; de-Paula,F.J.; Távora,P.F.; Brasileiro-Filho,G.; Villa,L.L.; Simpson,A.J.G.. |
| To assess the clinical relevance of a semi-quantitative measurement of human cytomegalovirus (HCMV) DNA in renal transplant recipients within the typical clinical context of a developing country where virtually 100% of both receptors and donors are seropositive for this virus, we have undertaken HCMV DNA quantification using a simple, semi-quantitative, limiting dilution polymerase chain reaction (PCR). We evaluated this assay prospectively in 52 renal transplant patients from whom a total of 495 serial blood samples were collected. The samples scored HCMV positive by qualitative PCR had the levels of HCMV DNA determined by end-point dilution-PCR. All patients were HCMV DNA positive during the monitoring period and a diagnosis of symptomatic infection was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymerase chain reaction; Human cytomegalovirus; Renal transplant; Diagnosis; Viral load. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200010 |
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| Andrade,Elisabete; Rocha,Daniele; Fontana-Maurell,Marcela; Costa,Elaine; Ribeiro,Marisa; de Godoy,Daniela Tupy; Ferreira,Antonio G.P.; Tanuri,Amilcar; Alvarez,Patrícia; Brindeiro,Rodrigo. |
| ABSTRACT The Brazilian Public Health Service provides freely αPEG-IFN to treat patients infected with HCV. The primary goal of HCV therapy is the long-term elimination of HCV from the blood to reduce the risk of HCV associated complications and death. Patient viremia affects the treatment duration and response, thus influencing clinical decisions. We developed a high-throughput method to perform the quantification of RNA hepatitis C virus (HCV) virus load in plasma samples to monitor patients under treatment. The method is based on a duplex detection, in a one-step real-time RT-PCR assay and it has been validated according to the rules established by the official Brazilian regulatory agency (ANVISA). This new method was compared to a commercial kit... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HCV; Viral load; Real Time PCR; Monitoring patients. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000500418 |
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| Ariede,Jovita Ramos; Pardini,Maria Inês de Moura Campos; Silva,Giovanni Faria; Grotto,Rejane Maria Tommasini. |
| Although HCV has hepatic tropism, the presence of the virus in extra-hepatic compartments has been well documented. Platelets have been described as carriers of the virus in the circulation and may be a natural reservoir for the virus. However, few studies have been performed to evaluate the levels of HCV RNA in plasma and platelets are equal or differ in some way. Therefore, the aim of this study was to perform a comparative evaluation of the stability of HCV RNA in plasma and isolated platelets. Four aliquots of whole plasma obtained from patients infected with HCV were incubated at 37 °C for 0, 48, 96 and 144 h. After incubation, the plasma and platelet pellet was obtained from each aliquot. Viral RNA in plasma and platelets was quantified by q-PCR. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis C Virus; Platelets; Viral load; Virological relapse. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200627 |
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| Zlotowski,P.; Corrêa,A.M.R; Castro,L.A; Koerich,P.K.V; Barcellos,D.E.S.N; Driemeier,D. |
| Relata-se a quantificação de PCV-2, por meio de PCR em tempo real, no trato gastrintestinal, linfonodo mesentérico, tonsilas e fezes de nove suínos refugo da fase de crescimento, provenientes de rebanhos com histórico de diarreia não responsiva ao tratamento com antibioticoterapia. Com base nos resultados histopatológicos e imunoistoquímicos, os animais foram classificados como afetados por PCV-2 (n=5) e não afetados (n=4). Foi observada diarreia em todos os animais do grupo afetado por PCV-2. Em todas as amostras testadas foi detectado PCV-2, no entanto, a carga viral observada nos tecidos, bem como a excreção nas fezes foi significantemente maior (P<0.01) nos animais com diarreia. A maior concentração de PCV-2 foi observada nos linfonodos... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: PCV-2; Viral load; Diarrhea. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600036 |
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| Gerber,P.F.; Garrocho,F.M.; Lobato,Z.I.P.. |
| The aim of this study was to characterize the porcine circovirus 2 (PCV2) infections in farrowing sows and to evaluate an association with piglet viremia and weight. Twenty sows and 100 newborn piglets were studied. Colostrum and serum of the sows were obtained on the day of parturition. Milk samples were collected on day 20 postpartum. Blood samples were taken and the piglets were weighed on days 1, 20, 42, 63 and 84 postpartum. Colostrum, milk and serum were evaluated for PCV2 DNA load. Serum was evaluated for neutralizing antibodies. PCV2 DNA was found in 17/20 serum samples, 14/20 colostrum samples and 11/20 milk samples. On day 1 postpartum 29% of piglets were viremic. PCV2 viral load ranged from 3.02 to 6.75 log10 copies/mL considering all sampled... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Porcine circovirus 2; Colostrum; Milk; Viral load; Weight. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200012 |
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| Registros recuperados: 16 | |
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